Table 1 Comparison of differences between Capn3-deficient and WT mice, as well as between male and female mice of area, Feret’s diameter, minimal Feret’s diameter and percentage of cells with centralized nuclei of myocytes in gastrocnemius, soleus and psoas muscle at different ages is presented.
1.5 months | 3 months | |||||||
|---|---|---|---|---|---|---|---|---|
WT or female | Capn3-deficient or male | 2-way ANOVA | WT or female | Capn3-deficient or male | 2-way ANOVA | |||
Gastrocnemius muscle | Area | Genotype | 2497 ± 525.1 | 2182 ± 543.5 | F (1, 11) = 1.629, p = 0.23 | 2275 ± 619.0 | 2112 ± 628.5 | F (1, 11) = 0.1250, p = 0.73 |
Gender | 2456 ± 566.2 | 2218 ± 528.9 | F (1, 11) = 1.036, p = 0.33 | 2056 ± 635.1 | 2363 ± 574.0 | F (1, 11) = 1.003, p = 0.34 | ||
Interaction | F (1, 11) = 1.044, p = 0.33 | F (1, 11) = 1.221, p = 0.29 | ||||||
Feret’s diameter | Genotype | 78.94 ± 10.36 | 77.56 ± 12.42 | F (1, 11) = 0.1286, p = 0.73 | 77.48 ± 11.29 | 74.88 ± 12.84 | F (1, 11) = 0.0787, p = 0.78 | |
Gender | 81.53 ± 9.109 | 75.29 ± 12.47 | F (1, 11) = 1.220, p = 0.29 | 72.35 ± 10.24 | 80.74 ± 12.31 | F (1, 11) = 1.848, p = 0.2 | ||
Interaction | F (1, 11) = 0.5307, p = 0.48 | F (1, 11) = 0.2712, p = 0.61 | ||||||
Minimal Feret’s diameter | Genotype | 46.06 ± 4.220 | 41.81 ± 5.249 | F (1, 11) = 3.090, p = 0.11 | 43.48 ± 6.543 | 41.22 ± 4.830 | F (1, 11) = 0.3826, p = 0.55 | |
Gender | 44.14 ± 5.623 | 43.49 ± 5.015 | F (1, 11) = 0.2059, p = 0.66 | 41.91 ± 6.986 | 43.02 ± 4.331 | F (1, 11) = 0.1819, p = 0.68 | ||
Interaction | F (1, 11) = 1.073, p = 0.32 | F (1, 11) = 2.065, p = 0.18 | ||||||
Central nuclei | Genotype | 0.02168 ± 0.02474 | 0.01570 ± 0.03507 | F (1, 26) = 0.2946, p = 0.59 | 0.007911 ± 0.01729 | 0.03347 ± 0.04007 | F (1, 26) = 10.47, p = 0.0033 | |
Gender | 0.02450 ± 0.03995 | 0.01323 ± 0.01821 | F (1, 26) = 0.9542, p = 0.34 | 0.007667 ± 0.01425 | 0.03375 ± 0.04124 | F (1, 26) = 10.82, p = 0.0029 | ||
Interaction | F (1, 26) = 0.5372, p = 0.47 | F (1, 26) = 6.220, p = 0.0193 | ||||||
Soleus muscle | Area | Genotype | 1417 ± 525.3 | 1323 ± 222.8 | F (1, 11) = 0.9223, p = 0.36 | 1119 ± 189.5 | 1176 ± 318.2 | F (1, 11) = 0.3266, p = 0.58 |
Gender | 1583 ± 419.8 | 1178 ± 228.2 | F (1, 11) = 8.007, p = 0.0164 | 1104 ± 215.3 | 1193 ± 293.6 | F (1, 11) = 0.6274, p = 0.45 | ||
Interaction | F (1, 11) = 4.324, p = 0.06 | F (1, 11) = 1.502, p = 0.25 | ||||||
Feret’s diameter | Genotype | 56.38 ± 10.08 | 57.68 ± 6.214 | F (1, 11) = 0.005412, p = 0.94 | 51.64 ± 3.785 | 52.96 ± 9.384 | F (1, 11) = 0.3184, p = 0.58 | |
Gender | 61.45 ± 8.577 | 53.25 ± 5.258 | F (1, 11) = 6.038, p = 0.0318 | 50.75 ± 4.812 | 53.98 ± 8.508 | F (1, 11) = 1.157, p = 0.31 | ||
Interaction | F (1, 11) = 2.943, p = 0.11 | F (1, 11) = 2.205, p = 0.17 | ||||||
Minimal Feret’s diameter | Genotype | 35.26 ± 6.272 | 33.08 ± 3.157 | F (1, 11) = 1.943, p = 0.19 | 31.70 ± 2.808 | 31.51 ± 2.985 | F (1, 11) = 0.001794, p = 0.97 | |
Gender | 36.60 ± 5.260 | 31.91 ± 3.285 | F (1, 11) = 6.494, p = 0.0271 | 31.23 ± 3.132 | 32.04 ± 2.505 | F (1, 11) = 0.2993; p = 0.6 | ||
Interaction | F (1, 11) = 3.395, p = 0.09 | F (1, 11) = 0.3039, p = 0.59 | ||||||
Central nuclei | Genotype | 0.01315 ± 0.01594 | 0.009549 ± 0.01103 | F (1, 26) = 1.142, p = 0.3 | 0.007642 ± 0.009844 | 0.08989 ± 0.1168 | F (1, 26) = 9.888, p = 0.0041 | |
Gender | 0.01844 ± 0.01551 | 0.004918 ± 0.006987 | F (1, 26) = 10.12, p = 0.0038 | 0.03043 ± 0.05271 | 0.06384 ± 0.1174 | F (1, 26) = 2.327, p = 0.14 | ||
Interaction | F (1, 26) = 0.08909, p = 0.77 | F (1, 26) = 2.570, p = 0.12 | ||||||
Psoas muscle | Area | Genotype | 2649 ± 731.9 | 2554 ± 651.1 | F (1, 11) = 0.1108, p = 0.75 | 4221 ± 1070 | 3528 ± 678.0 | F (1, 10) = 2.117, p = 0.18 |
Gender | 2481 ± 761.0 | 2701 ± 604.8 | F (1, 11) = 0.2462, p = 0.63 | 3978 ± 1094 | 3771 ± 814.0 | F (1, 10) = 0.3805, p = 0.55 | ||
Interaction | F (1, 11) = 1.980, p = 0.19 | F (1, 10) = 0.5522, p = 0.47 | ||||||
Feret’s diameter | Genotype | 77.84 ± 12.65 | 75.48 ± 10.90 | F (1, 11) = 0.1844, p = 0.68 | 98.23 ± 11.98 | 85.30 ± 10.70 | F (1, 10) = 4.232, p = 0.07 | |
Gender | 73.99 ± 13.12 | 78.85 ± 9.948 | F (1, 11) = 0.4623, p = 0.51 | 92.27 ± 11.54 | 91.26 ± 14.89 | F (1, 10) = 0.2029, p = 0.66 | ||
Interaction | F (1, 11) = 1.563, p = 0.24 | F (1, 10) = 0.5154, p = 0.49 | ||||||
Minimal Feret’s diameter | Genotype | 48.30 ± 6.539 | 46.18 ± 5.413 | F (1, 11) = 0.5805, p = 0.46 | 61.46 ± 8.548 | 54.16 ± 6.400 | F (1, 10) = 3.167, p = 0.11 | |
Gender | 46.27 ± 7.125 | 47.96 ± 4.830 | F (1, 11) = 0.1552, p = 0.7 | 58.70 ± 10.21 | 56.92 ± 6.236 | F (1, 10) = 0.4408, p = 0.52 | ||
Interaction | F (1, 11) = 1.966, p = 0.19 | F (1, 10) = 0.2044, p = 0.66 | ||||||
Central nuclei | Genotype | 0.005767 ± 0.01214 | 0.08506 ± 0.1056 | F (1, 26) = 7.510, p = 0.0109 | 0.008009 ± 0.02078 | 0.2446 ± 0.1184 | F (1, 24) = 60.27, p < 0.0001 | |
Gender | 0.03809 ± 0.08121 | 0.05677 ± 0.09228 | F (1, 26) = 0.6065, p = 0.44 | 0.1189 ± 0.1386 | 0.1338 ± 0.1590 | F (1, 24) = 2.512, p = 0.13 | ||
Interaction | F (1, 26) = 0.8251, p = 0.37 | F (1, 24) = 1.296, p = 0.27 | ||||||
5 months | 10 months | |||||||
|---|---|---|---|---|---|---|---|---|
WT or female | Capn3-deficient or male | 2-way ANOVA | WT or female | Capn3-deficient or male | 2-way ANOVA | |||
Gastrocnemius muscle | Area | Genotype | 2220 ± 607.9 | 1821 ± 343.1 | F (1, 12) = 2.693, p = 0.13 | 2267 ± 570.3 | 2309 ± 582.5 | F (1, 11) = 7.819e-005, p = 0.99 |
Gender | 2192 ± 644.5 | 1849 ± 309.8 | F (1, 12) = 1.983, p = 0.18 | 2299 ± 528.5 | 2272 ± 627.4 | F (1, 11) = 0.0574, p = 0.81 | ||
Interaction | F (1, 12) = 0.3922, p = 0.54 | F (1, 11) = 3.974, p = 0.07 | ||||||
Feret’s diameter | Genotype | 77.32 ± 8.776 | 67.81 ± 8.032 | F (1, 12) = 5.120, p = 0.043 | 78.56 ± 11.44 | 75.70 ± 11.56 | F (1, 11) = 0.4597, p = 0.51 | |
Gender | 75.53 ± 10.55 | 69.60 ± 7.858 | F (1, 12) = 1.989, p = 0.18 | 77.20 ± 10.17 | 77.26 ± 13.07 | F (1, 11) = 0.0344, p = 0.86 | ||
Interaction | F (1, 12) = 0.02785, p = 0.87 | F (1, 11) = 4.268, p = 0.06 | ||||||
Minimal Feret’s diameter | Genotype | 42.48 ± 6.027 | 39.39 ± 3.361 | F (1, 12) = 1.605, p = 0.23 | 42.55 ± 4.682 | 44.21 ± 4.652 | F (1, 11) = 0.3204, p = 0.58 | |
Gender | 42.53 ± 6.232 | 39.35 ± 2.908 | F (1, 12) = 1.700, p = 0.22 | 43.56 ± 4.180 | 43.06 ± 5.328 | F (1, 11) = 0.08736, p = 0.77 | ||
Interaction | F (1, 12) = 0.3146, p = 0.59 | F (1, 11) = 2.841, p = 0.12 | ||||||
Central nuclei | Genotype | 0.01593 ± 0.02070 | 0.02458 ± 0.03047 | F (1, 28) = 1.033, p = 0.32 | 0.02981 ± 0.05312 | 0.08141 ± 0.1091 | F (1, 26) = 4.573, p = 0.0421 | |
Gender | 0.01137 ± 0.01410 | 0.02914 ± 0.03209 | F (1, 28) = 4.352, p = 0.0462 | 0.02207 ± 0.03264 | 0.09026 ± 0.1131 | F (1, 26) = 7.259, p = 0.0122 | ||
Interaction | F (1, 28) = 2.719, p = 0.11 | F (1, 26) = 1.655, p = 0.21 | ||||||
Soleus muscle | Area | Genotype | 1003 ± 174.9 | 892.4 ± 126.8 | F (1, 12) = 2.160, p = 0.17 | 1253 ± 128.4 | 987.5 ± 186.3 | F (1, 11) = 11.22, p = 0.0065 |
Gender | 1006 ± 195.3 | 889.7 ± 88.58 | F (1, 12) = 2.373, p = 0.15 | 1089 ± 190.6 | 1175 ± 225.2 | F (1, 11) = 0.5505, p = 0.47 | ||
Interaction | F (1, 12) = 0.02650, p = 0.87 | F (1, 11) = 1.862, p = 0.2 | ||||||
Feret’s diameter | Genotype | 49.42 ± 4.438 | 45.60 ± 3.074 | F (1, 12) = 3.833, p = 0.07 | 54.52 ± 2.500 | 48.45 ± 5.232 | F (1, 11) = 8.220, p = 0.0153 | |
Gender | 48.65 ± 5.216 | 46.38 ± 2.707 | F (1, 12) = 1.357, p = 0.27 | 51.66 ± 5.300 | 51.71 ± 4.978 | F (1, 11) = 0.06033, p = 0.81 | ||
Interaction | F (1, 12) = 0.03386, p = 0.86 | F (1, 11) = 0.8403, p = 0.38 | ||||||
Minimal Feret’s diameter | Genotype | 29.87 ± 2.298 | 28.01 ± 1.931 | F (1, 12) = 2.967, p = 0.11 | 33.25 ± 2.653 | 28.82 ± 2.578 | F (1, 11) = 13.89, p = 0.0033 | |
Gender | 29.59 ± 2.764 | 28.30 ± 1.547 | F (1, 12) = 1.433, p = 0.25 | 29.81 ± 2.886 | 32.74 ± 3.468 | F (1, 11) = 4.607, p = 0.055 | ||
Interaction | F (1, 12) = 0.07462, p = 0.79 | F (1, 11) = 1.604, p = 0.23 | ||||||
Central nuclei | Genotype | 0.01200 ± 0.02526 | 0.1204 ± 0.08926 | F (1, 28) = 20.79, p < 0.0001 | 0.02629 ± 0.05243 | 0.1652 ± 0.1526 | F (1, 26) = 12.71, p = 0.0014 | |
Gender | 0.07480 ± 0.08504 | 0.05759 ± 0.08650 | F (1, 28) = 0.5243, p = 0.48 | 0.1418 ± 0.1550 | 0.03317 ± 0.05497 | F (1, 26) = 8.448, p = 0.0074 | ||
Interaction | F (1, 28) = 0.03181, p = 0.86 | F (1, 26) = 2.740, p = 0.11 | ||||||
Psoas muscle | Area | Genotype | 3919 ± 933.6 | 3515 ± 859.3 | F (1, 12) = 0.9524, p = 0.35 | 4689 ± 828.9 | 3097 ± 1199 | F (1, 10) = 7.438, p = 0.0213 |
Gender | 3302 ± 845.5 | 4132 ± 771.7 | F (1, 12) = 4.014, p = 0.06 | 3781 ± 1437 | 4004 ± 1229 | F (1, 10) = 5.823e-005, p = 0.99 | ||
Interaction | F (1, 12) = 0.4274, p = 0.53 | F (1, 10) = 0.9112, p = 0.36 | ||||||
Feret’s diameter | Genotype | 97.08 ± 14.54 | 86.44 ± 12.37 | F (1, 12) = 2.938, p = 0.11 | 109.8 ± 20.38 | 76.95 ± 14.00 | F (1, 10) = 9.926, p = 0.0103 | |
Gender | 85.24 ± 12.36 | 98.27 ± 13.39 | F (1, 12) = 4.408, p = 0.0576 | 92.54 ± 28.66 | 94.43 ± 18.29 | F (1, 10) = 0.03379, p = 0.86 | ||
Interaction | F (1, 12) = 0.1425, p = 0.71 | F (1, 10) = 0.06675, p = 0.8 | ||||||
Minimal Feret’s diameter | Genotype | 56.44 ± 6.550 | 52.70 ± 9.821 | F (1, 12) = 0.8461, p = 0.38 | 71.32 ± 14.86 | 47.70 ± 9.484 | F (1, 10) = 10.13, p = 0.0098 | |
Gender | 51.30 ± 8.816 | 57.85 ± 6.717 | F (1, 12) = 2.592, p = 0.13 | 59.20 ± 20.36 | 59.92 ± 13.51 | F (1, 10) = 0.009501, p = 0.92 | ||
Interaction | F (1, 12) = 0.1457, p = 0.71 | F (1, 10) = 0.03838, p = 0.85 | ||||||
Central nuclei | Genotype | 0.01370 ± 0.02551 | 0.1967 ± 0.08265 | F (1, 28) = 66.91, p < 0.0001 | 0.04103 ± 0.05051 | 0.2748 ± 0.08950 | F (1, 24) = 74.14, p < 0.0001 | |
Gender | 0.1043 ± 0.1056 | 0.1062 ± 0.1192 | F (1, 28) = 0.007105, p = 0.93 | 0.1495 ± 0.1267 | 0.1692 ± 0.1586 | F (1, 24) = 0.5029, p = 0.49 | ||
Interaction | F (1, 28) = 0.00093, p = 0.98 | F (1, 24) = 1.543, p = 0.23 | ||||||
15 months | ||||||||
|---|---|---|---|---|---|---|---|---|
WT or female | Capn3-deficient or male | 2-way ANOVA | ||||||
Gastrocnemius muscle | Area | Genotype | 2165 ± 430.1 | 1762 ± 322.6 | F (1, 10) = 6.958, p = 0.0248 | |||
Gender | 2135 ± 519.1 | 1792 ± 215.0 | F (1, 10) = 5.439, p = 0.0419 | |||||
Interaction | F (1, 10) = 1.065, p = 0.33 | |||||||
Feret’s diameter | Genotype | 77.73 ± 6.821 | 67.98 ± 6.476 | F (1, 10) = 12.00, p = 0.0061 | ||||
Gender | 75.83 ± 9.251 | 69.88 ± 6.141 | F (1, 10) = 5.754, p = 0.0374 | |||||
Interaction | F (1, 10) = 0.1110, p = 0.75 | |||||||
Minimal Feret’s diameter | Genotype | 41.69 ± 4.096 | 38.32 ± 3.204 | F (1, 10) = 4.247, p = 0.067 | ||||
Gender | 41.28 ± 4.965 | 38.73 ± 2.282 | F (1, 10) = 2.802, p = 0.13 | |||||
Interaction | F (1, 10) = 1.038, p = 0.33 | |||||||
Central nuclei | Genotype | 0.02932 ± 0.02536 | 0.08908 ± 0.05544 | F (1, 24) = 12.79, p = 0.0015 | ||||
Gender | 0.05978 ± 0.06138 | 0.05863 ± 0.04326 | F (1, 24) = 0.1966, p = 0.66 | |||||
Interaction | F (1, 24) = 0.1562, p = 0.7 | |||||||
Soleus muscle | Area | Genotype | 1533 ± 224.3 | 1174 ± 208.5 | F (1, 10) = 8.891, p = 0.0138 | |||
Gender | 1360 ± 325.5 | 1347 ± 251.0 | F (1, 10) = 0.2845, p = 0.61 | |||||
Interaction | F (1, 10) = 0.4569, p = 0.51 | |||||||
Feret’s diameter | Genotype | 60.84 ± 3.879 | 55.02 ± 8.206 | F (1, 10) = 2.474, p = 0.15 | ||||
Gender | 57.84 ± 8.047 | 58.02 ± 6.112 | F (1, 10) = 0.03170, p = 0.86 | |||||
Interaction | F (1, 10) = 0.1758, p = 0.68 | |||||||
Minimal Feret’s diameter | Genotype | 36.40 ± 2.938 | 29.49 ± 1.847 | F (1, 10) = 26.60, p = 0.0004 | ||||
Gender | 32.75 ± 5.208 | 33.14 ± 3.563 | F (1, 10) = 0.2060, p = 0.66 | |||||
Interaction | F (1, 10) = 1.234, p = 0.29 | |||||||
Central nuclei | Genotype | 0.03763 ± 0.04982 | 0.2598 ± 0.08783 | F (1, 24) = 64.76, p < 0.0001 | ||||
Gender | 0.1691 ± 0.1189 | 0.1283 ± 0.1474 | F (1, 24) = 0.1122, p = 0.74 | |||||
Interaction | F (1, 24) = 1.556, p = 0.22 | |||||||
Psoas muscle | Area | Genotype | 2165 ± 430.1 | 1762 ± 322.6 | F (1, 10) = 6.958, p = 0.0248 | |||
Gender | 2135 ± 519.1 | 1792 ± 215.0 | F (1, 10) = 5.439, p = 0.0419 | |||||
Interaction | F (1, 10) = 1.065, p = 0.33 | |||||||
Feret’s diameter | Genotype | 102.3 ± 9.992 | 77.10 ± 12.14 | F (1, 10) = 17.77, p = 0.0018 | ||||
Gender | 87.29 ± 14.91 | 92.13 ± 19.55 | F (1, 10) = 0.04516, p = 0.84 | |||||
Interaction | F (1, 10) = 2.222, p = 0.17 | |||||||
Minimal Feret’s diameter | Genotype | 63.78 ± 6.670 | 48.58 ± 9.332 | F (1, 10) = 11.30, p = 0.0072 | ||||
Gender | 55.81 ± 12.14 | 56.56 ± 10.89 | F (1, 10) = 0.1006, p = 0.76 | |||||
Interaction | F (1, 10) = 0.8664, p = 0.37 | |||||||
Central nuclei | Genotype | 0.06754 ± 0.07212 | 0.2797 ± 0.08263 | F (1, 24) = 63.22, p < 0.0001 | ||||
Gender | 0.1607 ± 0.1225 | 0.1866 ± 0.1446 | F (1, 24) = 4.288, p = 0.0493 | |||||
Interaction | F (1, 24) = 1.406, p = 0.25 | |||||||
