Table 2 Percentage of myocytes with centralized nuclei in gastrocnemius muscle (a), soleus muscle (b) and psoas muscle (c) of male and female Capn3-deficient and WT mice at different ages and a simplified scoring system are presented. Percentages up 5% in gastrocnemius muscle until the age of 15 months, soleus and psoas muscle until the age of 10 months, and up 10% in aged soleus and psoas muscle (15 months), respectively, are classified as normal. 5–15% are classified as slightly affected, 15–25% are classified as moderately affected, > 25% are classified as severely affected.
Age | WT | Capn3-deficient | |||||
|---|---|---|---|---|---|---|---|
Female | Male | Normal | Female | Male | p | Score | |
(a) Gastrocnemius muscle | |||||||
1.5 months | 2.33% | 2.05% | 0–5% | 2.54% | 0.60% | 0.59 | |
3 months | 0.43% | 1.15% | 0–5% | 1.10% | 6.34% | 0.0033 | 5–15% |
5 months | 1.41% | 1.78% | 0–5% | 0.87% | 4.05% | 0.32 | |
10 months | 1.02% | 4.94% | 0–5% | 3.39% | 14.47% | 0.0421 | 5–15% |
15 months | 2.12% | 3.54% | 0–5% | 8.87% | 8.96% | 0.0015 | 5–15% |
(b) Soleus muscle | |||||||
1.5 months | 2.2% | 0.66% | 0–5% | 1.59% | 0.32% | 0.3 | |
3 months | 0.87% | 0.65% | 0–5% | 5.21% | 14.02% | 0.0041 | 5–15% |
5 months | 1.8% | 0.55% | 0–5% | 13.11% | 10.97% | < 0.0001 | 5–15% |
10 months | 4.86% | 0.39% | 0–5% | 23.50% | 7.21% | 0.0014 | 5–15-25% |
15 months | 6.25% | 1.90% | 0–10% | 24.91% | 27.41% | < 0.0001 | > 25% |
(c) Psoas muscle | |||||||
1.5 months | 0.79% | 0.42% | 0–5% | 6.07% | 10.94% | 0.0109 | 5–15% |
3 months | 0.00% | 1.4% | 0–5% | 20.8% | 29.35% | < 0.0001 | 15–25% |
5 months | 1.31% | 1.43% | 0–5% | 19.55% | 19.8% | < 0.0001 | 15–25% |
10 months | 4.74% | 3.26% | 0–5% | 25.16% | 30.58% | < 0.0001 | > 25% |
15 months | 5.35% | 7.81% | 0–10% | 24.1% | 33.13% | < 0.0001 | > 25% |
