Table 2 Parameter values for the template of the iFCM data analysis.

From: Beyond growth: comparative impact of plastics and natural particles on EPS dynamics and aggregation in Rhodomonas Salina

Region

Objects

Intensity Ch011

Width (µm)

Length (µm)

R3

Non-attached bacteria

0–50,000

0–4

0–4

R4

Aggregates including bacteria only

0–50,000

> 4

> 4

R7

Single celled R. salina

> 50,000

6–12.5

0–20

R8

Aggregates including R. salina cells and bacteria

> 50,000

> 12.5

> 20

  1. To determine the amount of bacteria in R4 and the amount of bacteria and algal cells in R8, Ch02 and Ch011 *.tif images of the objects in both regions were exported using the Ideas software (v.6.2.187.0) and subsequently analysed applying a custom made macro in ImageJ (v1.54 m, https://imagej.net/ij).