Fig. 5

The effect of DPM on protein degradation of APOA1. CCD-8Lu and Hs68 cells were plated at a density of 4,000 cells/cm² in a 60 mm dish and incubated with or without 400 µg/mL DPM for 24 h at 70% confluence. (A) The mRNA level of APOA1 was determined by RT-qPCR analysis. (B) Western blot analysis of APOA1. CCD-8Lu and Hs68 cells were incubated with or without 400 µg/mL DPM. 100 µg/mL CHX was added after 12 h of DPM treatment, and then treated cells were harvested simultaneously at 24 h of DPM treatment. (C) Western blot analysis of APOA1. CCD-8Lu and Hs68 cells were incubated with or without 400 µg/mL DPM. 20 µM MG132 was treated for 6 h before cells were harvested at 24 h of DPM treatment. Each value is the mean ± SEM of three independent experiments. * p < 0.05. ns, not significant.