Fig. 4
From: A nile red fluorescence assay for LC3 autophagy protein binding to lipid bilayers
A comparison of the Nile red fluorescence and the ultracentrifugation methods to estimate LC3 protein binding to lipid bilayers. Effects of CL and Cer. Data for LC3A, LC3B, and LC3 proteins are given. Top row, maximum observed values of % ROIR decrease. Middle row, slopes of the % ROIR vs. LC3 concentration curves. Bottom row, % bound protein, measured with an ultracentrifugation-flotation method10. Bilayer lipid compositions were: 1, eSM: DOPE (50:50); 2, eSM: DOPE: eCer (40:50:10); 3, eSM: DOPE: CL (33:33:33); 4, eSM: DOPE: CL: eCer (23:33:33:10). Average values ± S.D., n = 3. Statistical significance was estimated according to the Student’s t-test, p values are indicated for each pair of significantly different data: ** p < 0.01, *** p < 0.001.
