Table 1 Primer sequences, amplicon sizes, and annealing temperatures for all genes analyzed by quantitative real-time PCR (qRT-PCR). GAPDH was used as the housekeeping gene for normalization.
Gene | Forward primer | Reverse primer | Product size (bp) | Annealing temp (°C) |
|---|---|---|---|---|
miR-155 | TTAATGCTAATCGTGATAGGGGT | ATATGTAGGAGTCAGTTGGAGGC | 55 | 61 |
TP53 | CATAGTGTGGTGGTGCCCTATGAG | CAAAGCTGTTCCGTCCCAGTAGA | 172 | 63 |
AIFM1 | CAGAAAAAGGCCGCGTTATCT | ATACAATCAGTACCCTGGCCCC | 160 | 59 |
CASP3 | TTCCCAGGTTTTGTTTCCTG | CCTTTCACCGAAACAGCATT | 143 | 61 |
BCL2 | CTGCACCTGACGCCCTTCACC | CACATGACCCCACCGAACTCAAAGA | 119 | 61 |
BAX | CGGGTTGTCGCCCTTTTCTA | TGGTTCTGATCAGTTCCGGC | 82 | 60 |
mTOR | GCCCAGGCCGCATTGTCTCTAT | GCAGTAAATGCAGGTAGTCATCCAGGTT | 84 | 62 |
LC3A | GCCTTCTTCCTGCTGGTGAAC | AGCCGTCCTCGTCTTTCTCC | 91 | 58 |
GAPDH (housekeeping) | TGCACCACCAACTGCTTAGC | GGCATGGACTGTGGTCATGAG | 87 | 59 |
