Fig. 3

EV flow cytometry results. Group means are shown as lines, and whiskers represent the SEM. One-way ANOVA was used to compare the different treatments. Asterisks represent significant changes with p-values < 0.05 (*), < 0.01 (**), < 0.001 (***) and < 0.0001 (****). (a) Percentages of positive events for CFSE and PanEV staining (EVs, left) and additionally for EGFR staining (EGFR + EVs, right). (b) Number of particles that were positive for CFSE and PanEV staining (EVs, left) and additionally for EGFR staining (EGFR + EVs, right), normalized to the volume of cell culture supernatant used for EV concentration.