Table 3 Sequences and information of the RT-qPCR primers used for validation of the final eight biomarkers. fw = forward, rv = reverse, bp = base pairs.
Target | Primer sequences (5’—> 3’) | Source | Tm | Amplicon size |
|---|---|---|---|---|
ARF1 | – | BioRad (qHsaCED0045157) | 60 °C | – |
UBC | fw: CTGGTGCTCCGTCTTAGAGG rv: TTTCCCAGCAAAAGATCAACC | Designed with Primer3 | 60 °C | 169 bp |
GAPDH | fw: CAGCCTCAAGATCATCAGCA rv: GTCTTCTGGGTGGCAGTGAT | Designed with Primer3 | 58 °C 60 °C | 135 bp |
NDRG1 | fw: GACCTGGAGATTGAGCGACC rv: CCCAACCACCAACAGAGCA | Designed with Primer3 | 60 °C 60 °C | 75 bp |
ISG20 | fw: GCCTCCTACACAAGAGCATC rv: CCCTCGCATCTTCCACCG | Designed with Primer3 | 61 °C 60 °C | 64 bp |
FOXM1 | fw: AGCGACAGGTTAAGGTTGAGG rv: TCTCAGTGCTGTTGATGGCG | Designed with Primer3 | 60 °C 61 °C | 120 bp |
TPX2 | fw: ATGATGCCCCCTCGGATTTC rv: AGCCCTCCAGTTCCATTCTTC | Designed with Primer3 | 60 °C 60 °C | 133 bp |
MYBL2 | fw: CCTGCCTTACAAGTGGGTGG rv: ACCAAGCATCAGGGTCCGA | Designed with Primer3 | 61 °C 61 °C | 107 bp |
AHNAK2 | fw: GGAAGCGAGATTCAGGGGAC rv: GGACAGCCTCTGGACGACT | Designed with Primer3 | 60 °C 61 °C | 60 bp |
GPRC5C | fw: GATGCACAAAGTTCCGTCCG rv: AGTACATGTCTTCAGCCCGC | Designed with Primer3 | 60 °C 60 °C | 113 bp |
THBD | fw: GGGGTGATTAGAGGGAGGAGA rv: TTGCCCAGTGGTCCAGTGA | Designed with Primer3 | 60 °C 61 °C | 65 bp |
