Fig. 3 | Scientific Reports

Fig. 3

From: Exploring PKMYT1 as a potential marker for colorectal cancer progression through bioinformatics analyses and experimental validation

Fig. 3

PKMYT1 maintained the cell proliferation of CRC in vitro. (A) IHC for PKMYT1 in the tumor and adjacent samples; (B) qPCR assay evaluated the mRNA levels of PKMYT1 in normal and CRC cell lines; qPCR (C) and Western blot (D) assay determined the efficiency of PKMYT1 knockdown by siRNAs. (E) The cell proliferation of CRC cells transfected with si-PKMYT1-1 were measured by CCK-8 assays. (F) Colony formation assays were conducted with or without PKMYT1 knockdown in CRC cells. (G) Histogram showed the number of colonies of CRC cells from the groups indicated, the results were analyzed statistically. (* P < 0.05, ** P < 0.01, *** P < 0.001). (H) Flow cytometry evaluation the effect of siPKMYT1 on the cell cycle of HCT116 and RKO cells.

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