Table 2 Calibration parameters of 05 bioactive markers.
Parameters | Name of the bioactive markers | ||||
|---|---|---|---|---|---|
Andrographolide (AG) | Piperine (PP) | Picroside-I (P-I) | Picroside-II (P-II) | Alpha-Cyprone (AC) | |
Linearity range (µg) | 0.5−5 | 0.5−5 | 0.1−1.0 | 0.1−1.0 | 0.1−1.0 |
Equation | y = 0.0026x + 0.0024 | y = 0.0048x + 0.0168 | y = 0.013x + 0.0019 | y = 0.0054x + 0.0002 | y = 0.0127x + 0.0013 |
LOD (µg) | 0.7682 | 0.847768548 | 0.1867 | 0.0690 | 0.1503 |
LOQ (µg) | 2.32787 | 2.5689956 | 0.5658 | 0.2091 | 0.4553 |
Regression coefficient (r2) | 0.9861 | 0.9830 | 0.9792 | 0.9971 | 0.9866 |
Rf | 0.563 ± 0.005 | 0.706 ± 0.015 | 0.28 ± 0.018 | 0.186 ± 0.012 | 0.803 ± 0.006 |
Inter-Day Precision (% RSD) | 0.45%−1.92% | 0.17%−1.52% | 0.85%−1.90% | 0.53%−1.87% | 0.50%−1.89% |
Intra-Day Precision (% RSD) | 0.86%−1.92% | 0.33% −0.90% | 0.51%−0.91% | 0.90%−1.91% | 0.75%−1.61% |
Accuracy (% recovery) | 95%−103% | 96%−98% | 98%−102% | 99%−102% | 97%−101% |
Wavelength (nm) | 229 | 343 | 279 | 264 | 254 |
