Table 4 Intra-day precision studies of the bioactive markers.

From: Simultaneous quantification of five bioactive markers for standardization of ayurvedic polyherbal formulation Jwarahara Kwatha Choornam using HPTLC

Concentration (µg)

Intraday precision studies

Shift-1

Shift-2

Shift-3

Mean AUC (n = 5)

SD

% RSD

Mean AUC (n = 5)

SD

% RSD

Mean AUC (n = 5)

SD

% RSD

Andrographolide (AG)

2

0.008452

0.00015579

1.84323237

0.008374

0.000124

1.48077382

0.008432

0.000159

1.88567362

3

0.010834

0.00015241

1.40677497

0.01075

0.00013491

1.25497674

0.010714

0.0000929

0.86708979

4

0.01272

0.00024423

1.92004717

0.012526

0.00011238

0.89717388

0.013036

0.00013

0.99723842

Piperine (PP)

2

0.028474

0.000164

0.57596404

0.028374

0.000124

0.43701981

0.028434

0.0000961

0.33797566

3

0.032714

0.000166

0.50742801

0.032726

0.000159

0.48585223

0.032776

0.000162

0.4942641

4

0.036616

0.00016

0.43696745

0.036554

0.000185

0.50610056

0.036606

0.000333

0.90968694

Picroside-I (P-I)

0.4

0.007556

0.000137

1.81312864

0.00765

0.000146116

1.91001307

0.00753

0.00010693

1.42005312

0.6

0.010322

0.000189

1.8310405

0.010554

0.000132778

1.25808224

0.010362

0.00015123

1.45946728

0.8

0.01235

0.0000797

0.64534413

0.012632

0.000211234

1.67221343

0.01278

0.0000663

0.51877934

Picroside-II (P-II)

0.4

0.002456

0.0000385

1.56758958

0.00246

0.00003

1.2195122

0.002462

0.0000462

1.87652315

0.6

0.003492

0.0000559

1.60080183

0.003508

0.0000370135

1.05511688

0.003508

0.000067231

1.91650513

0.8

0.004634

0.0000472

1.01855848

0.004584

0.0000415933

0.9073582

0.004584

0.000069138

1.50824607

Alpha-Cyperone (AC)

0.4

0.00667

0.0000595819

0.89328186

0.006566

0.0000594138

0.90487055

0.006612

0.0000698

1.05565638

0.6

0.00943

0.0000728011

0.77201591

0.009496

0.000149766

1.57714827

0.009502

0.000113

1.18922332

0.8

0.011544

0.000132401

1.14692481

0.011504

0.0000873499

0.75930024

0.011624

0.000188

1.61734343

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