Table 5 Accuracy studies of the bioactive markers with the JKC formulation.

From: Simultaneous quantification of five bioactive markers for standardization of ayurvedic polyherbal formulation Jwarahara Kwatha Choornam using HPTLC

Name of the Marker

Amount of standard (µg) present in JKC sample (5 µL)

Amount of standard added (µg)

Theoretical added value

Amount of standard obtained (n = 3)

Recovery (%)

Average Recovery (%)

SD

% RSD

Andrographolide (AG)

3.63846454

0.5

4.13846454

4.2192308

101.952

103.408

1.59444

1.54189

4.35

105.111

4.2692308

103.16

1

4.63846454

4.3576923

93.9469

94.8866

1.04994

1.10652

4.3923077

94.6931

4.4538465

96.0198

1.5

5.13846454

4.8192308

93.7874

95.0598

1.65519

1.74121

4.8538462

94.461

4.9807692

96.9311

Piperine (PP)

3.36041667

0.5

3.86041667

3.7479167

97.0858

98.8847

1.55881

1.5764

3.8541667

99.8381

3.85

99.7302

1

4.36041667

4.1729167

95.7

96.6874

1.17939

1.2198

4.2020833

96.3688

4.2729167

97.9933

1.5

4.86041667

4.7083333

96.871

97.3711

0.49308

0.5064

4.7333333

97.3853

4.75625

97.8568

Picroside-I (P-I)

0.1426

0.1

0.2426

0.2438

100.5291

102.8542

2.2274

2.1656

0.2546

104.968

0.2510

103.066

0.2

0.3426

0.3412

99.2515

98.3533

1.7532

1.78316

0.3311

96.3324

0.3408

99.4761

0.3

0.4426

0.4423

99.9421

99.8841

0.9573

0.9584

0.4462

100.8110

0.4377

98.8992

Picroside-II (P-II)

0.602469

0.1

0.7025

0.7148

101.7571

99.9121

1.8453

1.8470

0.7019

99.9121

0.6889

98.0668

0.2

0.802469

0.8574

106.8461

102.1542

5.5688

5.4514

0.8314

103.6151

0.7704

96.0001

0.3

0.902469

0.8852

98.0848

99.3161

1.2312

1.2397

0.8962

99.3161

0.9074

100.5472

Alpha-Cyperone (AC)

0.21024

0.1

0.31024

0.3032

97.7145

98.0530

1.5508

1.5816

0.3101

96.6993

0.3095

99.7452

0.2

0.41024

0.4260

103.8381

101.5986

2.2080

2.1732

0.4079

99.4233

0.4165

101.5352

0.3

0.51024

0.4780

93.6721

97.1701

3.3325

3.4296

0.4976

97.5301

0.5118

100.3081

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