Table 6 Robustness studies of the bioactive markers.

From: Simultaneous quantification of five bioactive markers for standardization of ayurvedic polyherbal formulation Jwarahara Kwatha Choornam using HPTLC

Concentration (µg)

TLC Plate @ 20 min saturation

TLC Plate @ 30 min saturation

Mean AUC (n = 5)

SD

% RSD

Mean AUC (n = 5)

SD

% RSD

Andrographolide (AG)

2

0.008452

0.000156

1.843212

0.00842

0.000155

1.845638

3

0.010834

0.000152

1.406811

0.010764

0.000187

1.738787

4

0.01272

0.000244

1.920075

0.012658

0.000176

1.392533

Piperine (PP)

2

0.028474

0.000164

0.577397

0.02837

0.0000917

0.323058

3

0.032714

0.000166

0.506267

0.032664

0.000105

0.321528

4

0.036616

0.00016

0.437223

0.036658

0.000176

0.480841

Picroside-I (P-I)

0.4

0.007556

0.000137

1.816073

0.007544

0.000142

1.883037

0.6

0.010322

0.000189

1.82845

0.010486

0.00016

1.529713

0.8

0.01235

0.0000797

0.645238

0.012438

0.000185

1.48944

Picroside-II (P-II)

0.4

0.002476

0.000024083

0.97266515

0.002494

0.000039115

1.56837267

0.6

0.003468

0.0000497

1.433077

0.003443

0.0000416

1.209099

0.8

0.004612

0.000042661

0.92500993

0.0046

0.000027386

0.59535061

Alpha-Cyperone (AC)

0.4

0.00667

0.0000596

0.893282

0.006466

0.000116

1.798926

0.6

0.00943

0.0000728

0.772016

0.009542

0.0000898

0.94145

0.8

0.011544

0.000132

1.146924

0.011516

0.000167

1.451222

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