Fig. 2 | Scientific Reports

Fig. 2

From: Lactococcus lactis subsp. cremoris C60 promotes immunoglobulin a production from B cells through functional modification of dendritic cells in the intestinal environment

Fig. 2

Cytokine production assay in HK-C60-stimulated BMDCs. (A) Experimental design of BMDC stimulation assay with HK-C60. BMDCs were prepared by culturing with rmGM-CSF for 8 days (n = 5 for independent culture). The differentiated BMDCs were subjected to in vitro stimulation assay with TLR ligand or HK-C60. Used TLR ligands and target receptors are shown in table. (B-E) Cytokine production in BMDC stimulation assay. The BMDCs were cultured with vehicle, TLR ligand or HK-C60 at 37 °C for 24 h. The concentration of TNF-α (B), IL-6 (C), IL-12/IL-23p40 (D) and IL-10 (E) in the conditioned medium were measured by ELISA, respectively. The data are shown as mean ± SEM of five samples from two independent experiments. Student t-test or one-way ANOVA was used to analyze the data for significant differences. Values of *p < 0.05, **p < 0.01 and ***p < 0.001 were regarded as significant. ns: not significant.

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