Fig. 5

Molecular phylogenetic analysis of AOB genomes and amoA gene diversity. (a) A molecular phylogenetic tree based on 46 AOB genomes, obtained using an alignment-free method with JolyTree software. (b) Molecular phylogenetic analysis of amoA gene diversity in four AOB isolates. A maximum Likelihood tree was generated using the MEGA software based on 411 aligned nucleotide sites of the amoA gene. Maximum likelihood bootstrap values (%) were calculated with 1,000 replicates, with values above 50 shown at tree nodes. Accession numbers from DNA databases (DDBJ, EMBL, GenBank, and JGI) are indicated in square brackets. The assembly numbers for the 46 AOB genomes are provided in Table S1.