Table 1 Primer sequences of GOI and internal control gene used for qPCR analyses. In primer sequence column, F and R indicate the orientation (F: forward; R: reverse).
From: A multi-molecular biomarker assessment of thermal preconditioning in two scleractinian coral species
Species | Gene | Primer Sequence 5’ To 3’ | Reference |
|---|---|---|---|
P. damicornis | hsp70 | F: ATCCAGGCAGCGGTCTTGT | |
R: TCGAGCAGCAGGATATCACTGA | |||
Elongation Factor (ef) | F: CGCTGGCAAAGTGACAAAGG | ||
R: CAGACTTGCGATGAAATAGATAGGA | |||
S. pistillata | hsp70 | F: AGGCAACTCTCAACCCAAACA | |
R: AGGCAACTCTCAACCCAAACA | |||
18 s rRNA | F: AACGATGCCAACTAGGGATCA | ||
R: GGTTTCCCATAAGGTGCCAAA |
