Table 2 Primer pairs used in this study for complete whole-genome sequencing of HPV16.
From: Scalable long-read nanopore HPV16 amplicon-based whole-genome sequencing
Abbreviation | Name | Primer sequence (5′ → 3′) | Amplicon size (bps) | Start | Stop |
|---|---|---|---|---|---|
T1 | HPV16-31FO | CGTAACCGAAATCGGTTGAACC | 2151 | 31 | 52 |
HPV16-2160RE | CCAATCACCTCCATCATCTACC | 2181 | 2160 | ||
T2 | HPV16-1744FO | TGTCTAAACTATTATGTGTGTCTCCAATG | 3950 | 1745 | 1773 |
HPV16-5692RE | GATACTGGGACAGGAGGCAAGTAGACAGT | 5694 | 5666 | ||
T3 | HPV16-5133FO | TGGCATTAGGTACAGTAGAATTGG | 2658 | 5133 | 5156 |
HPV16-7769RE | ACTAGGGTGACATTTAGTTGGC | 7790 | 7769 | ||
U1 | HPV16-7110FO | CCTCATCTACCTCTACAACTGC | 1795 | 7110 | 7131 |
HPV16-973RE | CCTGTATCACTGTCATTTTCGTTCTC | 998 | 973 | ||
L1 | HPV16-31FO | CGTAACCGAAATCGGTTGAACC | 7760 | 31 | 52 |
HPV16-7769RE | ACTAGGGTGACATTTAGTTGGC | 7790 | 7769 |
