Table 1 Potential candidate peptides information among upregulated peptide in Hepatozoon canis infected group.

Potential peptide biomarkers identified by Maldi-TOF-MS

FRMD8

A0A8P0PM92

FERM domain-containing protein 8

AGTGEQGLLNAYCK

1423.79

2.72

8.579

N/A

N/A

CAB39L

A0A8P0SA42

Calcium binding protein 39 like

LLEFLSSFQKER

1495.26

2.10

8.642

N/A

N/A

STX18

A0A8I3ML77

Syntaxin-18

NNAGFRVWVLFFLVMCSFSLLFLD

2837.66

2.20

9.015

Intracellular protein ransport [GO:0006886]; Vesicle-mediated transport [GO:0016192]

Endoplasmic reticulum membrane [GO:0005789]

TRIM28

A0A8C0LTD6

Tripartite motif containing 28

HEPLVLFCESCDTLTCRDCQLNAHK

2874.77

2.38

8.68

N/A

Transcription corepressor activity [GO:0003714]; Zinc ion binding [GO:0008270]

FNDC3B

A0A8C0SL00

Fibronectin type III domain containing 3B

GEEATFQISGLQTNTDYRFRVCVCR

2892.06

2.37

9.108

N/A

N/A

Potential peptide biomarkers identified by LC-MS

ZEB2

A0A8I3N483

Zinc finger E-box binding homeobox 2

AYYAMNMEPNSDELLKISIAVGLPQEFVK

3271.79

4.87

20.714

Negative regulation of transcription by RNA polymerase II

DNA binding, DNA-binding Transcription factor activity, Metal ion binding

TNS1

A0A8P0P322

Tensin 1

EGRPHFWAR

1155.28

3.43

17.22

N/A

Phosphoprotein Phosphatase activity

MTOR

A0A8C0Q917

Serine/threonine-protein kinase mTOR

CFLKLGEWQLNLQGINESTIPK

2531.95

3.76

17.195

Negative regulation of autophagy, Positive regulation of protein metabolic process, Regulation of cellular component organization

ATP binding, protein serine/threonine kinase activity, Protein-containing complex binding

THBS1

A0A8I3PPU8

Thrombospondin 1

FYVVMWKQVTQSYWDTNPTRAQGYSGLSVK

3541.00

3.46

20.599

Cell adhesion, Cell migration, Chronic inflammatory response, Engulfment of apoptotic cell, Immune response,

Calcium ion binding, Collagen V binding, Endopeptidase inhibitor activity, Fibrinogen binding, Protein homodimerization activity

THBS4

A0A8P0NB64

Thrombospondin 4

AFSGSSQSPDSIELR

1580.67

4.48

20.177

Cell adhesion, Positive regulation of cell division, Response to unfolded protein

Calcium ion binding, Growth factor activity

TNXB

A0A8P0SWE0

Tenascin XB

EVLGSSADGALLVSLDGLR

1872.11

2.49

19.59

N/A

N/A

CHD3

A0A8C0NX13

DNA helicase

CENDWRTSFPPGLCWGDRR

2295.53

4.43

19.33

Chromatin remodeling

ATP binding, DNA binding, Helicase activity, Hydrolase activity, Metal ion binding

LAMA5

A0A8C0TLC0

Laminin subunit alpha 5

AEAELLYADLRRGFEAFPELYWQAPPSYLGDR

3745.17

3.99

19.275

Cell adhesion, Regulation of cell adhesion, Regulation of cell migration, Regulation of embryonic development

Signaling receptor binding

LAMA2

A0A8P0SPP3

Laminin subunit alpha 2

ALAQGYYWSAPAPYLGNK

1970.21

3.20

19.002

Cell adhesion, regulation of cell adhesion, Regulation of cell migration, Regulation of embryonic development

Signaling receptor binding

ITGB3

A0A8I3PMU9

Integrin beta

CECGSCVCIQPGSYGDTCEKCPTCPDACTFK

3249.71

3.71

18.383

Apolipoprotein A-I-mediated signaling pathway, Apoptotic cell clearance, Cell adhesion mediated by integrin

C-X3-C chemokine binding, Extracellular matrix binding, Fibroblast growth factor binding

ACACA

A0A8C0P9A9

Acetyl-CoA carboxylase 1

AIIRHSDLVTKEASFEYLQNEGER

2806.08

4.28

17.885

Fatty acid biosynthetic process, Malonyl-CoA biosynthetic process

Acetyl-CoA carboxylase activity, ATP binding, Metal ion binding

TAF1

A0A8I3P7H0

Transcription initiation factor TFIID subunit

DAGYGEKSFFAPEEENEEDFQMK

2697.82

3.83

17.877

DNA-templated transcription initiation

DNA binding, Histone acetyltransferase activity, Protein serine/threonine kinase activity

FBN2

A0A8I3NQU3

Fibrillin 2

AWNKPCEPCPTPGTADFK

1962.23

2.62

17.799

Bone trabecula formation, Camera-type eye development, Embryonic eye morphogenesis, Embryonic limb morphogenesis

Calcium ion binding

MAST4

A0A8I3NHR5

non-specific serine/threonine protein kinase

AFISGAHPAQISAVSFVPLKALAGR

2508.95

3.24

17.053

N/A

ATP binding, magnesium ion binding, Protein serine/threonine kinase activity