Table 2 Primer details used in the trial.

From: Genetic variation of barley genotypes using morphological traits, amylose content, and molecular markers

	Primer name	Sequence	References	Chr
1	Bmag0211	F: ATTCATCGATCTTGTATTAGTCC	Ali et al.³³	1H
1	Bmag0211	R: ACATCATGTCGATCAAAGC	Ali et al.³³	1H
2	GBM1411	F: TGCTCTACAAACCGCTGATG	Gougerdchi et al.³⁴	1H
2	GBM1411	R: CGGAGTTCAGTTCAGACCGT	Gougerdchi et al.³⁴	1H
3	Bmac0213	F: ATGGATGCAA GACCAAAC	Elakhdar et al.³⁵	1H
3	Bmac0213	R: CTATGAGAGGTAGAGCAGCC	Elakhdar et al.³⁵	1H
4	GBM1468	F: GTTCCTTCTTTGCCTCCTCC	Gougerdchi et al.³⁴	2H
4	GBM1468	R: TCCATGGTCCTCTCCTTCAC	Gougerdchi et al.³⁴	2H
5	Bmac0134	F: CCAACTGAGTCGATCTCG	Ali et al.³³	2H
5	Bmac0134	R: CTTCGTTGCTTCTCTACCTT	Ali et al.³³	2H
6	Bmag0749	F: CGGATTCTTGAGTAGTCTCTG	Elakhdar et al.³⁵	2H
6	Bmag0749	R: GATCTGTTTTTGTAGAACATGC	Elakhdar et al.³⁵	2H
7	GBM1280	F: CTTCTTCTTCTTGTTGGGCG	Gougerdchi et al.³⁴	3H
7	GBM1280	R: AAGGGATCAGTTTGGTTCCC	Gougerdchi et al.³⁴	3H
8	scssr25691	F: ACGAGCTGATATCCCACGAG	Gougerdchi et al.³⁴	3H
8	scssr25691	R: TCCGAGCTTCTTATCTTTGG	Gougerdchi et al.³⁴	3H
9	Bmag0023	F: AACACAGACCTACGGGTC	Wang et al.³⁶	3H
9	Bmag0023	R: CATGAGATAGATCCAAGCAC	Wang et al.³⁶	3H
10	scssr20569	F: ATCGAGCACCTACGAACC	Gougerdchi et al.³⁴	4H
10	scssr20569	R: TTGCATAGCGGAAGTAATCC	Gougerdchi et al.³⁴	4H
11	HVLEU	F: TTGGAAGTGTACAGCAATGGAG	Misganaw et al.³⁷	5H
11	HVLEU	R: TGAAAGGCCCCACAAGATAG	Misganaw et al.³⁷	5H
12	Bmag0375	F: CCCTAGCCTTCCTTGAAG	Misganaw et al.³⁷	4H
12	Bmag0375	R: TTACTCAGCAATGGCACTAG	Misganaw et al.³⁷	4H
13	HvLOX	F: CAGCATATCCATCTGATCTG	Ferreira et al.³⁸	5H
13	HvLOX	R: ACACCCTTATTTATTGCCTTAA	Ferreira et al.³⁸	5H
14	Bmac0163	F: TTTCCAACAG AGGGTATTTA CG	Polgari et al.³⁹	5H
14	Bmac0163	R: GCAAAGCCCATGATACATACA	Polgari et al.³⁹	5H
15	HVM65	F: AGACATCCAAAAAATGAACCA	Ferreira et al.³⁸	6H
15	HVM65	R: TGGTAACTTGTCCCCCAAAG	Ferreira et al.³⁸	6H
16	Bmac0018	F: GTCCTTTACGCATGAACCGT	Gougerdchi et al.³⁴	6H
16	Bmac0018	R: ACATACGCCAGACTCGTGTG	Gougerdchi et al.³⁴	6H
17	Bmac0040	F: AGCCCGATCAGATTTACG	Ali et al.³³	6H
17	Bmac0040	R: TTCTCCCTTTGGTCCTTG	Ali et al.³³	6H
18	Bmag0135	F: ACGAAAGAGTTACAACGGATA	Backes et al.⁴⁰	7H
18	Bmag0135	R: GTTTACCACAGATCTACAGGTG	Backes et al.⁴⁰	7H
19	GBM1102	F: GGCATCTGCTCTTGATGACA	Gougerdchi et al.³⁴	7H
19	GBM1102	R: GGAGGAAAATAGGCACGGAT	Gougerdchi et al.³⁴	7H
20	EBmac0603	F: ACCGAAACTA AATGAACTAC TTCG	Ali et al.³³	7H
20	EBmac0603	R: TGCAAACTGTGCTATTAAGGG	Ali et al.³³	7H

Chr = Chromosome, F = Forward, R = Reverse.

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