Table 1 Tested parameters of Tephrosia apollinea and the methods.
Parameters | Methods |
|---|---|
Na and K | Na and k were determined by digesting 1 g of each sample with nitric and hydrochloric acid according to (AOAC 969.23) |
Ca, P, Mg, Zn, Mn, and Cu | These minerals were analyzed by digesting 0.5 g of each sample with nitic and HCl acid through microwave digestor for 1 h as per (AOAC 2015.01) |
Dry matter | Dry matter determination was performed by taking 5 g of each sample to the oven adjusted at 105° C for 3 h (AOAC 922.06). |
Crude protein | Crude protein was determined through Kjeldahl method by digesting 0.5 g of each sample with sulfuric acid, and Kjeldahl tablet for 1 h [30]. |
Crude fiber | This parameter was obtained through digesting 2 g of each sample with 1.25% sodium hydroxide and 1.25% of sulfuric acid for 30 min (AOAC 962.09). |
Ash | Ash content was performed by taking 2 g of each dried sample to the muffle furnace adjusted at 600° C for 2 h (AOAC 942.05). |
Total digestible nutrients | This parameter was determined based on the fat, protein, crude fiber, and non-volatile ether extract [31]. |
Tannins and fat | Tannins and fat contents were performed according to [18]. |
Total flavonoids | Flavonoids contents were assessed according to [32]. |
