Fig. 2

Co-transfection of WT and Δ Alu fluorescent constructs. (a) The top row shows the WT human F3 3′-UTR construct. For each of the fluorescent constructs the ectodomain has been replaced by either mCherry or mNeonGreen. The Alu element is highlighted by a red box in the 3′-UTR, and red dotted line indicates a deletion of the Alu element; (b–e) Fluorescent constructs were co-transfected into MDA-MB-231 cells. Images were acquired as a Z-stack using Plan-Apochromat 63x/1.40 Oil DIC M27 lens on Zeiss LSM-780 inverted confocal microscope. Representative of multiple images, shown as a Z-project. Scale bars, 5 μm. Images analysed on Fiji software (ImageJ). (b) mCh-WT and mNG-WT; (c) mCh-ΔAlu and mNG-ΔAlu; (d) mNG-WT and mCh-ΔAlu; and (e) mCh-WT and mNG-ΔAlu. Fluorophores encoded by the ΔAlu 3′-UTR F3 constructs localise to the cell surface more readily than those encoded by the WT 3’-UTR F3 constructs.