Fig. 3
From: Impact of an Alu insertion on the cellular localisation of tissue factor protein
The presence of the Alu element in the F3 3′-UTR affects its localisation within MDA-MB-231 cells. MDA-MB-231 cells were co-transfected with markers to identify specific organelles along with a fluorophore under the influence of the F3 3′-UTR with either the Alu element present (WT) or not (∆Alu). (a) cell membranes, (b) early endosomes, (c) Golgi, (d) nucleus, (e) endoplasmic reticulum. (i) Left columns represent organelle localisation; middle column, WT or ΔAlu F3; and the right column, the merged image (colocalisation) of F3, the organelle marker, and Hoechst 33342 to identify the nucleus (blue). Images were acquired as a Z-stack using Plan-Apochromat 63x/1.40 Oil DIC M27 lens on Zeiss LSM-780 inverted confocal microscope. Representative of multiple images. Scale bars, 5 μm. (ii) Pearson’s correlation coefficient of the colocalisation of the organelle marker with the fluorophore protein under the influence of either the WT or ΔAlu 3′-UTR are shown to the right. The number of cells analysed/the number of independent experiments is indicated on the x axis. Data are expressed as mean ± SEM and analysed using one-way ANOVA. *p < 0.05 **p < 0.01.
