Fig. 2
HFD-induced obesity exacerbates the progression of experimental colitis. (A) Body weight changes in mice fed a low-fat diet (LFD) or HFD for 10 wk following 2,4,6-trinitrobenzenesulfonic acid (TNBS) treatment. *P < 0.05, **P < 0.01 versus respective LFD or HFD; #P < 0.05, ##P < 0.01 versus LFD + TNBS; n = 5 each group. (B) Gross morphology of the colons on day 3 after TNBS treatment. (C) Clinical score of the colons on day 3 after TNBS treatment. *P < 0.05 versus LFD + TNBS; n = 5 each group. (D) Hematoxylin and eosin histology of the colons on day 3 after TNBS treatment (arrows point to the ulcerated area; magnification: × 50). (E) Histological score of the colons on day 3 after TNBS treatment. *P < 0.05 versus LFD + TNBS; n = 5 each group. (F) Real-time PCR quantitation of pro-inflammatory cytokines and chemokines in colonic mucosa on day 3 after TNBS treatment. *P < 0.05, **P < 0.01 versus LFD or HFD; #P < 0.05 versus LFD or LFD + TNBS; n = 5 each group.
