Fig. 4
Blockade of AT1 receptor signaling ameliorates experimental colitis induced by long-term HFD. (A) Workflow diagram. (B) Body weight changes in mice on HFD-TNBS following losartan treatment. *P < 0.05, **P < 0.01 versus respective HFD or HFD LST; #P < 0.05, versus HFD TNBS; n = 5 each group. (C) Gross morphology of the colons in mice following losartan treatment. (D) Clinical score of the colons in mice following losartan treatment. **P < 0.01 versus HFD TNBS; n = 5 each group. (E). Hematoxylin and eosin histology of the colons in mice following losartan treatment (arrows point to the ulcerated area; magnification: × 50). (F). Histological score of the colons in mice following losartan treatment. **P < 0.01 versus HFD TNBS; n = 5 each group. (G) Real-time PCR quantitation of pro-inflammatory cytokines and chemokines in colonic mucosa following losartan treatment. **P < 0.01, ***P < 0.001 versus respective HFD or HFD LST; #P < 0.05, versus HFD TNBS; n = 5 each group. (H) Real-time PCR quantitation of Agtr1, Ren and Agtr2 in colonic mucosa on day 3 after TNBS treatment. *P < 0.05, **P < 0.01 versus respective HFD or HFD LST; #P < 0.05, #P < 0.01 versus HFD TNBS; n = 5 each group.
