Fig. 2

Representative immunofluorescence images for molecular changes in the aorta.A through F, Representative images for CD31 (green) with (A) eNOS, (B) VE-cadherin, (C) CD68, (D) F4/80, (E) CD3 or (F) TGF-β1 (red), with cell nuclei counterstained with DAPI (blue). Mice were irradiated with 0 Gy (upper panels) or 5 Gy (middle and lower panels), and injected with MSCs (lower panels) or vehicle (upper and middle panels) at 6 h after irradiation. All images were taken at 4 weeks after irradiation. Boxed areas in the left panels (tiled images) are shown at higher magnification in the right panels. Scale bars are as indicated. G, Representative images for aortic cells with subcellular fragments. Mice were injected with MSCs (b) or vehicle (a) at 6 h after irradiation with 5 Gy. All images were taken at 4 weeks after irradiation. Boxed areas in the upper left panels (tiled images) are shown at higher magnification in other two panels, with the upper right panels further enlarged in the lower panels: the upper left panels are for VE-cadherin, CD31 and DAPI, the upper right panels for CD31 and DAPI, and the lower panels for DAPI. Closed and open arrowheads in the lower panels point to vascular endothelial cells with subcellular fragments in the tunica intima and vascular smooth muscle cells with subcellular fragments in the tunica media, respectively. Scale bars are as indicated. For quantitative data, see Fig. 4.