Fig. 1
Representative materials and devices utilized during rat orthotopic lung transplantation. (A) Donor procedure instruments, (B) Recipient-side microsurgical instruments adapted from standard cataract surgery kits; (C) Cuff components: 18G green catheter (1) for arterial anastomosis; 16G gray catheters (2) for venous and bronchial anastomoses; (D) Standard cuff design (1.4 mm body and 1.4 mm tail length); (E) Soft-bristled brush positioned in a Petri dish, providing protective cushioning during graft preparation; (F) Custom-designed endotracheal tube fashioned from a central venous catheter for atraumatic intubation; (G) Harvard Apparatus® Inspira ASV ventilator used for controlled mechanical ventilation of small animals; (H) Monitoring and support devices: (1,2) Pulse oximeter with rodent-adapted probe; (3) Electrocautery unit for intraoperative hemostasis; (4) Improvised respiratory mask repurposed from a clinical drainage funnel for postoperative oxygen delivery.
