Fig. 4
From: Aging alters genomic instability at endogenous mutation hotspots in mice
(adapted from Del Mundo et al., 2017). The estimated site of cleavage is marked by blue bracket. (B) H-DNA cleavage from young and aged brain WCE. (C) Quantification of cleavage products from brain WCE. (D) H-DNA Cleavage from young and aged spleen WCE. (E) Quantification of cleavage products from spleen WCE. Cleavage products marked by red arrows. M, DNA ladder. NTC, non-extract treated control. Error bars represent mean ± SEM, n = 3 mice, statistical significance determined using student’s t-test
Cleavage of H-DNA conformation with age. (A) R2-5’ radiolabeled oligo forming an H-DNA conformation.
