Fig. 4 | Scientific Reports

Fig. 4

From: Dexamethasone inhibits Mycobacterium tuberculosis-induced glycolysis but preserves antimicrobial function in primary human macrophages

Fig. 4

Dexamethasone reduces the production of cytokines in human MDM infected with Mtb. PBMC were isolated from buffy coats and MDM were adherence purified and differentiated for 7 days in 10% human serum. MDM were treated with VC (ethanol 0.02%) or dexamethasone (0.05, 0.5 or 5 µM) for 1 h prior to infection with Mtb (MOI 1–10). Supernatants were harvested 72 h post infection and the secretion of IL-1β (a), TNF-α (b), IL-6 (c), IL-8 (d) and IL-10 (e) was quantified by ELISA. Data are represented as means ± standard deviation. Statistical significance was determined using a two-way ANOVA with a Dunnett’s post-test *p ≤ 0.05, ** p ≤ 0.05, *** p ≤ 0.005, **** p ≤ 0.001 (n = 8).

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