Table 1 Primers used in real-time PCR.

From: Effects of vitamin D supplementation under different salinities on hybrid red tilapia growth performance, hormonal profile and growth genes expression

Gene

Primer sequence

Tm

Sequence reference

β-actin

F: CCACACAGTGCCCATCTACGA

R: CCACGCTCTGTCAGGATCTTCA

58

NCBI: NM_001009784.2

GH

F: CCAGCCATGAACTCAGGTAAGACA

R: TGCTGAGAGGAGACGCCCAAACA

62.7

64.2

M97765

IGF-II

F: TCCCCAGCTGGAAGATGTGTCACG

R: CTGGACGCAGCTGAAATCCTGTGG

61.4

54.5

AF033802

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