Fig. 2 | Scientific Reports

Fig. 2

From: Poly(A) tail dynamics, non-adenine incorporation and alternative polyadenylation shape the host transcriptome in COVID-19 pathogenesis

Fig. 2

Gene expression profiling of controls and COVID-19 patients. (A) The heatmap displays the normalised expression of 52 common genes for DRS and cDNA sequencing. Red colour indicates values greater than 0, while blue indicates values less than 0. (B) The scatter plot illustrates the relationship between log2FC values for the 52 DEGs identified in both sequencing methods. The x-axis represents log2FC values for cDNA Illumina sequencing, while the y-axis depicts log2FC values for DRS Nanopore sequencing. The red line highlights the Pearson correlation. The R value is displayed in the upper left corner. (C) The Venn diagram presents DEGs in cDNA Illumina (blue), DEGs in DRS Nanopore (green), and common DEGs (red). (D) DEGs characteristic of COVID-19 patients. The top panels correspond to DRS Nanopore, while the bottom panels represent cDNA Illumina sequencing. All DEGs are colored according to the Venn diagram. On the left, MA plots show the relationship between log2FC and log2 (Mean of normalised counts), while on the right, Volcano plots show the relationship between -log10(padj) and log2FC.

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