Table 4 System suitability testing parameters of the proposed HPLC method for SAC and VAL in spiked human plasma.
Parameters | Obtained value | Reference value [51] | ||
|---|---|---|---|---|
VAL | SAC | IBU | ||
Retention time, min | 2.570 | 3.948 | 6.461 | |
Asymmetry factor | 0.866 | 0.825 | 0.830 | 0.8–1.2 |
Retention factor, k | 0.474 | 1.264 | 2.704 | < 10 |
Resolution, Rs | 9.042 | 13.911 | > 1.5 | |
Selectivity, a | 2.669 | 2.140 | > 1 | |
Number of theoretical plates, N | 5175.136 | 9514.382 | 16798.450 | Increase with efficiency of the separation |
Height equivalent theoretical plates, H (cm/plate) | 0.0029 | 0.0016 | 0.0009 | The smaller the value the higher the column efficiency |
