Table 5 Robustness results of the proposed HPLC method for SAC and VAL in their pure forms.

From: A sensitive green HPLC-fluorescence method for simultaneous analysis of sacubitril and valsartan in pure forms, pharmaceutical dosage form and human plasma

Parameter

VAL

SAC

tR*

Rs**

N***

tR*

Rs**

N***

Flow rate (− 0.1, 0.9 mL/min)

2.511

8.261

4940.250

3.77

14.182

8675.789

Flow rate (+ 0.1, 1.1 mL/min)

2.632

9.967

5427.843

4.151

14.531

10517.965

Ethanol % (+ 2%, 62%)

2.546

8.241

5078.931

3.802

13.756

8823.695

Ethanol % (− 2%, 58%)

2.643

8.957

5473.308

4.008

14.636

9805.770

pH 2.6 (+ 0.1)

2.544

8.963

5070.955

3.91

14.708

9332.109

pH 2.4 (− 0.1)

2.601

9.127

5300.737

3.992

13.501

9727.637

  1. *Retention time, min.
  2. **Resolution factor (Rs).
  3. **Number of Theoretical Plates (N).
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