Table 8 Eco-Scale penalty points (PPs) for the determination of SAC and VAL using the proposed HPLC method.

From: A sensitive green HPLC-fluorescence method for simultaneous analysis of sacubitril and valsartan in pure forms, pharmaceutical dosage form and human plasma

Parameters

Penalty points

Reagents

Amount PPs

Hazard PPs (Pictograms x Signal word)

Total PPs

Pictograms

Signal word (warning/danger)

Ethanol

3

2

2

12

Methanol

2

3

2

12

Water

3

0

0

0

Potassium dihydrogen phosphate

1

0

1

0

Phosphoric acid

1

1

2

2

Instrument (HPLC)

Methods using 0.1–1.5 kWh per sample

1

Occupational hazard

Analytical process hermitization

0

Waste

Amount > 10 mL

5

No treatment

3

Total penalty points

S 34

Analytical Eco-Scale total score * = 100 − sum of penalty points

66

  1. *Analytical Eco-Scale score, ideal method if = 100, excellent > 75, acceptable > 50, inadequate < 50.