Fig. 4
Comparison of the different migration assays. (A) Comparison of scratch and zone-exclusion (Z-E) assays. Gap closure was measured relative to cell size using the mean area obtained from single-cell experiments, to avoid effects of the initial gap area on closure rates. (B) Z-score normalized comparison of 24-h migration metrics derived from single-cell MSD and velocity, scratch assay velocity, Z-E assay velocity, and transwell assay results. (C) Heat map showing relative cell line rankings across 24-hour migration metrics from single-cell MSD and velocity, scratch assay velocity, Z-E assay velocity, and transwell assay results.
