Fig. 8
Comparison of different migration assays under nintedanib treatment. (A) To assess the relative sensitivity of each assay, Inhibition% was calculated per cell line. The calculation was based on: 24 h MSD AUC values for single-cell tracking, relative scratch area AUC for scratch assay, relative gap area AUC values for zone-exclusion (Z-E) assay, and relative migration (treated/ control cell number) for transwell assay. Data represent the mean ± SEM of at least three independent experiments. Statistical analysis was performed using the Kruskal–Wallis test; p < 0.05 was considered statistically significant. (B) Classification accuracy of a machine learning-based random forest model trained to distinguish between control, 1 µM, and 2 µM nintedanib-treated cells across different migration assays (single-cell tracking, scratch, and Z-E assay). Data represent mean classification accuracy per cell line; error bars indicate 95% confidence intervals based on cross-validation.
