Fig. 5 | Scientific Reports

Fig. 5

From: A buffer-tuning strategy to profile domain-specific activity of chimeric I-TevI/CRISPR gene editors in vitro

Fig. 5

Cleavage of Tev CNNNG motifs decreases with distance from the TTTV PAM. Plot of the frequency of cleavage for mapped CNNNG motifs upstream (+ bp) of the TTTV PAM. Points are from four independent mapping experiments with targeted (\(\hbox {gRNA}_{\lambda }\)) or non-targeted (\(\hbox {gRNA}_\textrm{AAVS1}\)) guide RNAs. Shaded area indicates the 95% confidence interval for the fit of the data (non-parametric smoothing function).

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