Fig. 6
Cas12a cleavage at on-target and mismatched target sites is sensitive to salt concentration. (A) Schematic of the linear substrate with the on-target site flanked by an EcoRI site indicated. The position of mismatches to the on-target site are indicated above the sequence, and labeled by their position in the linear substrate shown below. The anticipated cleavage products and sizes are also indicated on the linear substrate. (B) Representative agarose gel of Cas12a cleavage under the indicated NaCl concentrations. Sizes and identity of products are indicated on the left of the gel. US, uncut substrate. (C) Plot of cleavage of on-target or mismatched target sites grouped by the position of the mismatches. Points are three independent replicates with the shaded area indicated the 95% confidence interval for fit of the data to a non-parametric smoothing function. Uncropped gel image for panel B is shown in Supplementary Fig. S11.
