Table 1 Observed reaction rates for targeted (\(k_{1}\) and \(k_{2}\)) and non-targeted (\(k_{obs}\)) cleavage of pJET-AAVS1 (nM DNA/sec) for all proteins at 20:1, except TevCas12a at 10:1 (*) protein:DNA.

From: A buffer-tuning strategy to profile domain-specific activity of chimeric I-TevI/CRISPR gene editors in vitro

Enzyme

\(k_{1}\)

\(k_{2}\)

\(k_{obs}\)

Cas12a

0.118 ± 0.016

0.035 ± 0.007

N.D.

TevCas12a*

N.D.

N.D.

0.112 ± 0.010

TevdCas12a

N.D.

N.D.

0.067 ± 0.009

dTevCas12a

0.053 ± 0.011

0.038 ± 0.011

N.D.

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