Fig. 5
Reversion of ebselen antiviral activity by phosphatidylinositol (PI). The imHC cells were infected with DENV-2 (A) or ZIKV (B) at an MOI of 1, and Vero cells were infected with EV71 (C) or CHIKV (D) at an MOI of 0.02 and 0.0025, respectively. After the virus inoculum was discarded, the cells were maintained in media containing various concentrations of ebselen, PI, a combination of ebselen and PI, or 0.5% DMSO as an untreated control. The viral supernatants of CHIKV were collected at 24 hpi, while those of DENV, ZIKV, and EV71 were collected at 48 hpi, followed by viral titer determination. The data are presented as the mean of triplicate experiments, and error bars represent the standard deviation. The differences between two groups were assessed using the independent samples t-test: ebselen alone vs. ebselen with 1.25 µg/ml PI, and ebselen alone vs. ebselen with 2.5 µg/ml PI (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ns = non-significant difference).
