Fig. 6
IMPA1 silencing inhibits virus production, and reduced IMPA1 expression partially impairs ebselen antiviral effect. imHC cells were seeded in a 24-well plate at a density of 6.5 × 105 cells/well. The following day, cells were transfected with siRNA targeting IMPA1 (si_IMPA1) or a non-targeting control siRNA (si_irrelevant). Two days post-transfection, cells were either mock-infected or infected with DENV-2 at a multiplicity of infection (MOI) of 2. After removal of the viral inoculum, cells were maintained in media with or without 20 µM ebselen for 24 h. Viral supernatants were collected for virus titer quantification, and cells were harvested for protein expression analysis via western blotting. (A) shows the virus titer of DENV-2 in infected cells transfected with either si_irrelevant or si_IMPA1, and treated with or without ebselen. (B) and (C) display representative protein bands for IMPA1, DENV-2 NS1, and GAPDH. Protein band intensities were quantified, normalized to GAPDH expression levels, and plotted as graphs: (D) relative IMPA1 expression levels and (E) relative DENV-2 NS1 expression levels. Data are presented as the mean of duplicate experiments. The differences between two groups were assessed using the independent samples t-test (*p ≤ 0.05; **p ≤ 0.01; p ≤ 0.001; ns = non-significant).
