Fig. 1

Representative images of Xenopus laevis embryos exposed to fluconazole (FLU). Embryos were treated from stage NF 3 to NF 45 with increasing concentrations of FLU (1, 100, and 1000 µg/L). Image acquisition and scoring followed FETAX criteria for morphological endpoints. Phenotypic abnormalities were observed at all tested concentrations, with most prominent features including reduced head size, eye deformities, craniofacial malformations, altered pigmentation, and gut abnormalities. Images were captured at NF stage 45 using a stereomicroscope. Scale bar: 500 µm.