Fig. 2

α-Mangostin neutralizes melanogenesis induced by H₂O₂. Cultured individual follicles were treated with different concentrations of α-mangostin with or without genotoxic stress (2% H₂O₂). Pigmented melanoblasts in bulge areas were examined under bright field microscopy. For example, blank control without H₂O₂ treatment, H₂O₂ treatment, and H₂O₂ with a positive control compound from Dr. David Fisher’s lab. Pigments were circled in red. B. Paired T-test demonstrates that the pigment-suppressive effects were much stronger with higher concentrations of α-mangostin. The error bar denotes the standard error of the mean (SEM). C. Representative immunofluorescence microscopic images of cells-stained ROS/DAPI for H₂O₂ induced ROS. Pigments are cycled in red. D. Image analysis-based ROS expression. ROS levels were evaluated under microscopy presented in panel C. n = 7, **P < 0.01, indicating a statistically significant difference between 2% H₂O₂ and α-mangostin (10 µM) + 2% H₂O₂ treatment.