Fig. 2

Expression patterns of selected genes. (A) Venn diagram showing the overlap among total no. of detected genes, and those from the libraries prepared for Egr1 DS genes (bioinformatically predicted and provided by authors¹⁸) and NS genes (literature search). (B) Venn diagram showing overlap among total no. of DEGs, and detected genes of Egr1 DS- and NS- libraries. (C and D) Heatmaps showing the temporal expression of Egr1 DS- and NS- library DEGs. The genes are separated into different categories based on literature review. In (C): Dop/EcR, dopamine-ecdysteroid signaling (the role of Ddc in ecdysteroid pathway in not yet ascertained and thus not included in the count of Dop/EcR signaling genes in main text); circa, genes involved in molecular circadian clock; NT, neurotransmission; IS, intracellular signaling; TF1, transcription factors; SY1, synaptic genes (most of them are involved in synaptic vesicle release); others, genes that could not be designated into a functional category. In (D): NTr, receptors of neurotransmitters; NPr, receptors of neuropeptides; BAr, receptors for biogenic amines; SM, second messenger cascade genes; TF2, transcription factors; SY2, synaptic genes (all of them are involved in synaptic vesicle release); NTe, neurotransmitter enzymes; NP, neuropeptide genes. Corresponding bar diagrams indicate that 89.74% Egr1 DS DEGs and 77.5% NS DEGs show peak expression at 10:00. (E) (i) Heatmap showing expression of all annotated canonical clock genes. Right panel: Cosinor plot showing expression of 5 clock genes characterized for whole brain in³⁷. (ii) Heatmap showing expression of clock-related neuropeptide receptors (predicted in¹⁸). See also Figure S2 and Table S1. In (E) only cry2 and ASTC-R are DEGs.