Fig. 1
From: Regulation of glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neurons
Gpc6 is co-expressed with Gde2 in the mouse cortex and regulated by Gde2 expression. (a) Heat map showing the expression of Gde2 (Gdpd5) in excitatory neurons in the mouse cortex (left) and the expression of Glypican family proteins and Wnt signaling components in Gde2-expressing cortical neurons (right). Each subpanel is scaled differently to highlight the range of expression levels. Data from the Allen Brain Atlas. L: layer. (b,c). Representative images of RNAscope probing for Gpc6 transcripts in coronal sections of the adult mouse brain. The boxed area of the motor cortex in (b) is shown in magnified view in (c). Scale bar: (b) = 500 μm; (c) = 100 μm. (d,e). Representative western blot images of surface biotinylation of DIV21 WT and Gde2KO primary cortical neurons (d) and graph quantifying the surface levels of furin-converted GPC6 normalized to the membrane protein, Na-K ATPase (e). WT vs. Gde2KO: **p = 0.0015. n = 4 WT, 5 Gde2KO. Welch’s t-test. All graphs: mean ± sem.
