Fig. 2 | Scientific Reports

Fig. 2

From: Anticancer effect of piperine, a black pepper compound, regulating apoptosis mediated through extracellular vesicles and cathepsin D in acute leukemia

Fig. 2

Combination of piperine and daunorubicin is exhibited as synergistic effect in NB4 and MOLT-4 cells. (A) NB4 and MOLT-4 cells were treated with IC50 of piperine, 0.2 µM of daunorubicin and combination of piperine and daunorubcin for 48 h and then cell viability of NB4 and MOLT-4 cells was analyzed using MTT assay. (B) A combination index plot of combination of piperine and daunorubicin was constructed using CompuSyn. Fa (fraction affected) indicated the effect of combination of piperine and daunorubicin of three independent experiments. CI indicated combination index of each independent experiment. “P” was a piperine compound and “D” was a daunorubicin compound. The data were represented as mean ± standard error of mean (S.E.M) (n = 3). The statistical comparison was performed using one-way ANOVA. *** p < 0.001 and **** p < 0.0001 were considered as significant difference.

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