Fig. 3

Functional parameters show improved hepatocellular functionality when stored with AP39: bile was collected through the perfusions and, at the end of experiment, was measured. (a) Production was diminished in SCS livers, however, a slight increase was observed in SCS + AP39 compared to SCS. (b) When measured for metabolic parameters, bile glucose was elevated SCS compared to Fresh, while no elevation was observed between Fresh and SCS + AP39. (c) When plotting bile production against bile glucose, a weak correlation of r² = 0.355 was observed, although this correlation is limited by the fact that several SCS livers did not produce enough bile for measurement. At the end of perfusion, biopsies were taken and snap frozen in liquid nitrogen, after which key metabolic parameters were measured using LC-MS. The ratio of (d) ATP: AMP and (e) ATP: ADP was not different between groups, however, the (f) energy charge was diminished in SCS compared to Fresh and SCS + AP39, while no difference was observed between these two groups. Asterisks denote statistical significance (ordinary one-way ANOVA with multiple comparisons test) *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Boxes, median and interquartile range. Whiskers, 5th − 95th percentile. Error bars of bar charts, mean ± s.e.m.