Fig. 5 | Scientific Reports

Fig. 5

From: UW supplementation with AP39 improves liver viability following static cold storage

Fig. 5

TUNEL staining shows decreased apoptosis in livers stored with AP39: formalin fixed samples were also stained with terminal deoxynucleotidyl transferase (TUNEL) stain, revealing double-strand DNA breaks, a marker of apoptosis, in brown. (a) SCS + AP39 showed a marked reduction in TUNEL positive staining compared to SCS, although still elevated when compared to Fresh. (b) Following quantification, SCS without AP39 showed a greatly increased percentage of apoptotic positive cells compared to SCS + AP39. Asterisks denote statistical significance (ordinary one-way ANOVA with multiple comparisons test) *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Boxes, median and interquartile range. Whiskers, 5th − 95th percentile.

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