Fig. 1 | Scientific Reports

Fig. 1

From: Inner membrane components of the plasmid pKM101 type IV secretion system TraE and TraD are DNA-binding proteins

Fig. 1

TraE binds ssDNA and dsDNA. EMSA analysis with 50 nM of 16 nt pT ssDNA (A) and 50 nM of 16 bp pT/pA dsDNA (B) in the presence of TraE gradient from 100–1900 nM. (C) FP mP signal of 10–22 nt pT ssDNA and 10–22 bp pT/pA dsDNA (D) as a function of TraE protein concentration. Representation of the dissociation constant KD as a function of 10–22 length ssDNA (E) or 10–22 length dsDNA (F). Summary of TraE KD and R2 of each pT ssDNA (G) and each pT/pA dsDNA (H). Each experiment was independently repeated three times, and each value represent the mean of those experiments.

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