Fig. 3
TraD binds ssDNA and dsDNA. EMSA analysis with 50 nM of 14 nt pT ssDNA (A) and 50 nM of 14 bp pT dsDNA (B) in the presence of TraD gradient from 200–3800 nM. FP mP signal of 10–22 nt pT ssDNA (C) and 10–22 bp pT dsDNA (D) as a function of TraD protein concentration. Representation of the dissociation constant KD as a function of 10–22 length ssDNA (E) or 10–22 length dsDNA (F). Summary of TraD KD and R2 of each pT ssDNA (G) and each pT/pA dsDNA (H). Each experiment was independently repeated three times, and each value represent the mean of those experiments.
