Table 1 Design of experiment variables using BBD.
HAPNPs | DZHAPNPs | ||||||
|---|---|---|---|---|---|---|---|
Independent variables | Levels | Independent variables | Levels | ||||
-1 | 0 | +1 | -1 | 0 | +1 | ||
Factor 1: pH of ammonium phosphate solution | 8 | 10 | 12 | Factor 1: Ratio of the drug to HAPNPs | 1:1 | 1:2 | 1:3 |
Factor 2: pH of calcium nitrate solution | 8 | 11 | 14 | Factor 2: Volume of dispersion medium | 30 ml | 40 ml | 50 ml |
Factor 3: Dilution of the reactant solutions keeping the Ca/P ratio at 1:0.67 | 0.5 | 0.75 | 1 | Factor 3: Loading Time | 12 h | 18 h | 24 h |
Dependent variables | Constraints | Dependent variables | Constraints | ||||
Response 1: Particle Size (nm) | Minimum | Response 1: Particle Size (nm) | Minimum | ||||
Response 2: PDI | Minimum | Response 2: Entrapment Efficiency | Maximum | ||||
Response 3: Zeta Potential | Maximum | ||||||
